Protocols for Macroalgae Research
Bénédicte Charrier, Thomas Wichard, C R K Reddy
April 17, 2018
Published by CRC Press
Reference - 526 Pages - 53 Color & 68 B/W Illustrations
ISBN 9781498796422 - CAT# K30375
Summary
Protocols for Macroalgae Research describes state-of-the-art approaches and applications in seaweed research. The book is comprised of 31 protocols in all and provides critical information regarding the cultivation and preservation of seaweeds, their chemical composition, as well as their cellular and molecular characterization. This type of research is critical and timely, as the last few decades have witnessed remarkable advancements in phycological research worldwide. Consideration of algal resources for food, fuel, chemicals, feed, and fertilizer has furthered the development of diverse laboratory techniques that not only advance their utilization substantially, but also provided new insights in understanding the molecular basis of their form and function. This recent progress has enabled researchers to determine and quantify various metabolites and organic macromolecules such as carbohydrates, proteins, and lipids.
Protocols for Macroalgae Research thus uses macroalgae as a model organism for addressing fundamental research problems. No recent comprehensive literature of this format exists on this topic. As such, this state-of-the-art protocol text both develops and standardizes the various macroalgae approaches located within the field. It does so by featuring a wide-range of protocols from invited experts; each protocol features a brief introductory overview, an inventory of materials and reagents, and step-by-step experimental procedures, which include helpful notes offering care and cautions. This protocol book is an invaluable reference for beginning and preexisting researchers within the field of macroalgae.
Table of Contents
Section I: Cultivating and preserving seaweeds
Chapter 1 Seaweed in high-energy environments: Protocol
to move Saccharina cultivation offshore
Bela H. Buck and Britta Grote
Chapter 2 Cultivation protocol for Saccharina latissima
Silje Forbord, Kristine Braaten Steinhovden,
Kaia Kjølbo Rød, Aleksander Handå, and Jorunn Skjermo
Chapter 3 Derivation of clonal stock cultures and hybridization
of kelps: A tool for strain preservation and breeding
programs
Inka Bartsch
Chapter 4 Cryopreservation of macroalgae
John G. Day
Chapter 5 Unraveling seaweeds bacteriomes: From field site
to computer screen
Tânia Aires, Gerard Muyzer, Ester A. Serrão, and
Aschwin H. Engelen
Chapter 6 Heavy metal ecotoxicity on the early life history
stages of macroalgae
Pablo P. Leal and Michael Y. Roleda
Chapter 7 A simple protocol for a rapid and consistent
production of a large number of viable protoplasts
from the Ulvophycean species
Vishal Gupta and C.R.K. Reddy
Chapter 8 Purification of sporulation and swarming inhibitors
from Ulva: Application in algal life-cycle controlling
Ralf W. Kessler, Taghreed Alsufyani, and Thomas Wichard
Chapter 9 Preparation of axenic cultures in Ulva (Chlorophyta)
Gianmaria Califano and Thomas Wichard
Section II: Chemical composition
Chapter 10 Biochar production from seaweeds
Loretto Contreras-Porcia, Matías Araya,
Elizabeth Garrido-Ramírez, Cristian Bulboa,
Jean Pierre Remonsellez, Javier Zapata, Camila Espinoza,
and Jorge Rivas
Chapter 11 Identification and quantification of laminarins
in brown algae
Angelika Graiff, Wolfgang Ruth, and Ulf Karsten
Chapter 12 Determination of carbohydrate composition
of macroalgae
Wouter J.J. Huijgen, E.M. Cobussen-Pool, B.F. van Egmond,
and J.W. van Hal
Chapter 13 Quantification of proteins in seaweeds
Carl Safi, Jelle van Leeuwen, Yvette Telleman,
Nicole Engelen-Smit, Lambertus van den Broek,
and Paulien Harmsen
Chapter 14 Comprehensive phytohormone quantification
in the red alga Pyropia yezoensis by liquid
chromatography-mass spectrometry
Takakazu Matsuura, Izumi C. Mori, Yoko Ikeda,
Takashi Hirayama, and Koji Mikami
Chapter 15 Total phenolic content and antioxidant capacity
analysis of seaweed biomass
Xiaoru Hou, Randi Neerup, and Anne-Belinda Bjerre
Chapter 16 Extraction of phycocyanin and phycoerythrin pigments
Stewart William Beattie, Michèle Morançais, Paul Déléris,
Joël Fleurence, and Justine Dumay
Chapter 17 Quantification and localization of reactive oxygen
species in marine macrophytes
Manoj Kumar, Loretto Contreras-Porcia, Nirali M. Kumar,
and Peter J. Ralph
Chapter 18 Metabolomics of intra- and extracellular metabolites
from micro- and macroalgae using GC-MS
and LC-MS
Constanze Kuhlisch, Gianmaria Califano, Thomas Wichard,
and Georg Pohnert
Chapter 19 Preparative extraction of exometabolites
from seaweed surfaces
Florian Weinberger
Chapter 20 Disruption-free solid-phase extraction of surface
metabolites from macroalgae
Emilio Cirri and Georg Pohnert
Section III: Cellular and molecular characterization
Chapter 21 The immunodetection and in situ imaging of
cell-wall polysaccharides in brown algae
Amandine Siméon, Delphine Duffieux, Cécile Hervé,
Sophie Le Panse, Paul Knox, and Thomas Torode
Chapter 22 Atomic force microscopy based analysis of cell-wall
elasticity in macroalgae
Thomas Torode, Marina Linardic, J. Louis Kaplan,
and Siobhan A. Braybrook
Chapter 23 Dynamic and microscale mapping of cell growth:
Case of Ectocarpus filament cells
Hervé Rabillé, Bernard Billoud, Elodie Rolland,
and Bénédicte Charrier
Chapter 24 Actin fluorescent staining in the filamentous brown
alga Ectocarpus siliculosus
Hervé Rabillé, Maria Koutalianou, Bénédicte Charrier,
and Christos Katsaros
Chapter 25 Cryofixation of brown algae for transmission
electron microscopy
Chikako Nagasato, Christos Katsaros, and Taizo Motomura
Chapter 26 Probing the subcellular topography of
seaweeds: Transmission electron microscopy,
immunocytochemistry, and correlative light
microscopy
Sandra C. Raimundo and David S. Domozych
Chapter 27 Coralline algae preparation for scanning electron
microscopy and optical microscopy
- Kaleb, G. Alongi, and A. Falace
Chapter 28 Extraction of high quality RNA from brown algae
for transcriptomic analysis
Sandra Heinrich
Chapter 29 Induction of sexual reproduction in Spirogyra
cultures for laser capture microdissection of gametes
and zygotes
Denis Saint-Marcoux and Jane A. Langdale
Chapter 30 Cloning and expression strategies for the
postgenomic analysis of brown algae
Agnès Groisillier
Chapter 31 Polyethylene glycol-mediated transformation in
the green macroalga Ulva mutabilis (Chlorophyta):
A forward genetics approach
Jens Boesger, Michiel Kwantes, and Thomas Wichard