Jan Frieder Mohr

Research project

This project aims to identify novel metallophores based on recently developed LC/MS analytical techniques and to unravel their multiple functions particularly in nitrogen-fixing and microbial iron-cycling systems. To mimic natural sources of molybdenum, which is an essential cofactor of nitrogenases, we will develop libraries of synthetic chelating agents for molybdenum. The resulting complexes serve as “Mo-buffers” to control the availability of molybdenum species in aqueous cultures. To model redox shuttle systems in communities of Fe(II) oxidizers and Fe(III) reducers appropriate ligands as well as metal complexes will be developed.

Publications

Mohr, J.F., Gama, S.*, Roy, S.,  Bellenger J.-B., Plass, W.*, Wichard, T.* (2022) Hydroxypyridinones in nitrogen-fixing bacterial cultures: a metal buffer for molybdenum and simulation of natural conditions. Metallomics 14: mfac055. https://doi.org/10.1093/mtomcs/mfac055External link

Mohr, J.F., Baldeweg, F., Deicke, M.,  Morales-Reyes, C.F., Hoffmeister, D.,  Wichard, T.* (2021) Frankobactin metallophores produced by nitrogen-fixing Frankia Actinobacteria function in toxic metal sequestration Journal of Natural Products. 84: 1216-1225. DOI: 10.1021/acs.jnatprod.0c01291External link

Mohr, J.F., Weiss, A., Roy, S., Wichard, T.* (2020). Genome sequence of Frankia sp. strain CH37, a metallophore-producing, nitrogen-fixing actinobacterium isolated from the sea buckthorn, Hippophae rhamnoides (Elaeagnaceae). Microbiology Resource Announcement 9: e01184-20. https://doi.org/10.1128/MRA.01184-20External link

Ghaderiardakani F., Califano G., Mohr J.F., Abreu M.H., Coates J.C., Wichard T* (2019) Analysis of algal growth- and morphogenesis-promoting factors in an integrated multi-trophic aquaculture system for farming the green seaweed Ulva spp. Aquaculture Environment Interactions 11: 375-391. https://doi.org/10.3354/aei00319 External link

Kügler, S., Cooper, R.,E., Wegner, C.-E., Mohr, J.F., Wichard, T., Küsel, K.* (2019) Iron-organic matter complexes accelerate microbial iron cycling in an iron-rich fen. Science of Total Environment 646: 972-988.External link

Hermenau, R., Keishi, I., Gama, S., Hoffmann, B., Pfeifer-Leeg, M., Plass, W., Mohr, J.F., Wichard, T., Saluz H.,P., Hertweck, C.* (2018) Gramibactin is a bacterial siderophore with a diazeniumdiolate ligand system. Nature Chemical Biology. Online https://doi.org/10.1038/s41589-018-0101-9.External link

Baumeister, T. U. H., Ueberschaar, N., Schmidt-Heck, W., Mohr J. F., Deicke M., Wichard, T.*, Guthke R., Pohnert, G.* (2018) DeltaMS: A tool to track isotopologues in GC- and LC-MS data. MetabolomicsExternal link 14:41.External link

Deicke, M., Mohr, J. F., Bellenger, J. P., Wichard, T. (2014) Metallophore mapping in complex matrices by metal isotope coded profiling of organic ligands. Analyst, 23, 6096 - 6099External link.